玉米超氧化物歧化酶超声波提取技术的研究

以玉米湿法加工淀粉副产物玉米胚芽为原料,经超临界CO2萃取脱脂、低温超细粉碎处理后超声波破碎提取超氧化物歧化酶(SOD)。实验结果表明,最佳超声波提取工艺条件为固液比1:25、超声波处理时间6s、次数40次、间隔时间2s;采用热变性和丙酮沉淀及SephadexG-75葡聚糖凝胶过滤纯化后得到的SOD样品酶活收率为40.56%,蛋白去除率99.36%,比活力为3079.41U/mg,纯化倍数达到63.09;紫外波长扫描的结果表明其最大紫外吸收峰在260nm处,说明玉米胚SOD类型为Cu/Zn-SOD。

Study on Technology of Ultrasonic-assisted Extraction of Superoxide Dismutase from Corn

With corn embryo of coproduct from corn starch produced with wet method as material, superoxide dismutase(SOD) was extracted through sonication after supercritical CO2 fluid extraction for defating and low temperature crushing. The results showed that optimum conditions are solid to liquid 1:25, the time 6 s, the times 40 and the interval time 2 s. The crude extract was purified through thermal denaturation and acetone sediment and Sephadex G-75 column chromatography. The enzyme obtained activity recovery was 40.56%, the removal rate of protein was 99.36%, the specific activity was 3079.41 U/mg, and pure the purify multiple was 63.09. The scanning results of ultraviolet spectrophotometer showed that the peak of ultraviolet absorption of SOD from corn embryo was at 260 nm, the same to standard preparation. It was that the superoxide dismutase pure of corn embryo is Cu/Zn-SOD.