Elastomeric nanocomposite based on carbon nanotubes for Polymerase Chain Reaction device |
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Authors: | M. Quaglio S. BiancoR. Castagna M. Cocuzza C.F. Pirri |
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Affiliation: | a Center for Space Human Robotics, Fondazione Istituto Italiano di Tecnologia, Corso Trento 21, Turin 10129, Italy b Materials and Microsystem Lab (Chilab)-Latemar Unit, Materials Science and Chemical Engineering Department, Politecnico di Torino, Corso Duca degli Abruzzi 24, Turin 10129, Italy c CNR-IMEM, Parco Area delle Scienze 37a, Parma 43124, Italy |
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Abstract: | The low thermal conductivity of polymers is the main problem to design efficient disposable polymeric Polymerase Chain Reaction (PCR) devices. The introduction of nanocomposite (NC) materials to fabricate Lab-On-a-Chips (LOCs) for on-chip PCR can improve the thermal response of the final device, fastening the PCR thermocycling. In this study, we propose the application of the nanocomposite obtained introducing Multi-Walled Carbon NanoTubes (MWCNTs) into Poly(DiMethyl)Siloxane (PDMS). We demonstrate a better thermal behavior for devices made of the nanocomposite material, observing faster transitions and a better control in steady-state temperatures. The better thermal performance of the nanocomposite-based device is mirrored by an efficiency increase in PCR reaction, demonstrating the direct enhancing effect of the CNTs content in the composite. This novel approach allows us to reach a more precise control in the biological reaction, with a beneficial effect on process efficiency, actually introducing a new class of devices. |
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Keywords: | Poly(DiMethyl)Siloxane Carbon nanotubes Nanocomposite Polymerase Chain Reaction |
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