|
|||||
|
|
In vivo electrochemical impedance measurement on single cell membrane |
|
| Authors: | Seoung-Jai Bai Fritz B. Prinz |
| Affiliation: | a Department of Mechanical Engineering, Dankook University, Yong-in 448-701, Republic of Korea b Nanoscale Prototyping Laboratory for Energy Conversion and Storage, Department of Mechanical Engineering, Stanford University, Stanford, CA 94305, USA c Department of Material Science and Engineering, Stanford University, Stanford, CA 94305, USA |
| Abstract: | The electrochemical characteristics of the plasma membranes of single cells and organelles were investigated. Silicon fabrication technology was used to produce a metal ultra-microelectrode (UME). Furthermore, the UME was characterized in a cell medium using electrochemical impedance spectroscopy (EIS). A single rat fibroblast cell, or chloroplast purified from Peperomia metallica leaves, was immobilized by a micropipette after which the UME was inserted into its cytosolic space through cell membrane using a piezo actuator. An in vivo EIS measurement between the UME and the counter electrode outside of a single cell was taken. The measurements were analyzed using equivalent circuits in order to estimate the membrane impedance of a single cell. |
| Keywords: | Electrochemical impedance spectroscopy (EIS) Cell plasma membrane Single cell measurement Ultra-microelectrode (UME) |
| 本文献已被 ScienceDirect 等数据库收录！ | |