Microfluidic arrays for bioimprint of cancer cells |
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Authors: | Volker Nock Lynn MurrayFahmi Samsuri Maan M AlkaisiJohn J Evans |
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Affiliation: | a The MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Electrical and Computer Engineering, University of Canterbury, Private Bag 4800, Christchurch, New Zealand b Christchurch School of Medicine and Health Sciences, University of Otago, Christchurch 8140, New Zealand |
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Abstract: | We report the development and characterization of a microfluidics-based bioimprint process using high-density microchannel arrays for cell-culture and polymer delivery. The tubeless PDMS arrays consist of multiple independent microchannels and allow for parallelized bioimprint via automated dispensing and passive pumping. Using the microchannels, a 400 nm thin test pattern was replicated into a methacrylate biopolymer to demonstrate process applicability. Bioimprints of cobalt chloride stimulated Ishikawa endometrial cancer cells exhibiting exocytosis-like pore structures were compared with controls using AFM to exemplify a process application. The devices can be used for high-throughput cell assays, cell developmental studies and the formation of phenotype-specific biomimetic scaffolds. |
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Keywords: | Bioimprint Microchannel array Passive pumping AFM |
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