| 潲水油聚合酶链式反应鉴定技术研究 |
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| 引用本文： | 杨冬燕,杨小柯,杨永存,吴丽明,张慧敏,张倩,张艳炜,耿艺介,黄达娜,吴双,邓平建.潲水油聚合酶链式反应鉴定技术研究[J].中国食品卫生杂志,2011,23(3):255-260. |
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| 作者姓名： | 杨冬燕 杨小柯 杨永存 吴丽明 张慧敏 张倩 张艳炜 耿艺介 黄达娜 吴双 邓平建 |
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| 作者单位： | 深圳市疾病预防控制中心,广东,深圳,518020 |
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| 基金项目： | 深圳市科技计划项目(200902081); 广东省医学科研课题(A2009605) |
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| 摘 要： | 目的建立特异的潲水油聚合酶链式反应(PCR)鉴定方法。方法以猪肉、牛肉、羊肉、鸡肉、哺乳动物等动物源性基因及大米物种特异性基因作为靶基因,采用PCR及荧光PCR方法鉴定潲水油。结果 6个自制的粗制潲水油样品全部被鉴定为潲水油,7个疑似潲水油样品中的5个被鉴定为潲水油,而4个正常食用植物油全部被鉴定为非潲水油。结论以潲水油中可能混杂的动物源性基因和大米基因作为靶标,采用普通PCR或荧光PCR方法可有效鉴定潲水油。
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| 关 键 词： | 潲水油 动物源性基因 大米物种特异性基因 聚合酶链式反应 |
| 收稿时间： | 2010/8/23 0:00:00 |
Identification of hogwash oil with polymerase chain reaction |
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| Yang Dongyan,Yang Xiaoke,Yang Yongcun,Wu Liming,Zhang Huimin,Zhang Qian,Zhang Yanwei,Geng Yijie,Huang Dan,Wu Shuang and Deng Pingjian.Identification of hogwash oil with polymerase chain reaction[J].Chinese Journal of Food Hygiene,2011,23(3):255-260. |
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| Authors: | Yang Dongyan Yang Xiaoke Yang Yongcun Wu Liming Zhang Huimin Zhang Qian Zhang Yanwei Geng Yijie Huang Dan Wu Shuang and Deng Pingjian |
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| Affiliation: | Yang Dongyan,Yang Xiaoke,Yang Yongcun,Wu Liming,Zhang Huimin,Zhang Qian,Zhang Yanwei,Geng Yijie,Huang Dana,Wu Shuang,Deng Pingjian(Shenzhen Center for Disease Control and Prevention,Guangdong Shenzhen 518020,China) |
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| Abstract: | Objective To develop a specific polymerase chain reaction(PCR) method for identification of hogwash oil.MethodsGene sequences specific for pork,beef,mutton,chicken,mammal and rice-specific gene were used as targets for identification of hogwash oil by PCR and fluorescent PCR methods.ResultsSix home-made crude swill oil samples were all identified as hogwash oil,5 in 7 suspected swill oil samples were identified as hogwash oil,and no hogwash oil was detected in 4 normal edible vegetable oil samples.Conclusio... |
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| Keywords: | Hogwash oil animal-specific gene rice-specific gene polymerase chain reaction |
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