首页 | 本学科首页   官方微博 | 高级检索  
     


Vanadate inhibition of mitochondrial respiration and H+ ATPase activity in Saccharomyces cerevisiae
Authors:G E Henderson  I H Evans  I J Bruce
Affiliation:School of Biological Sciences and Environmental Health, Thames Polytechnic, London, U.K.
Abstract:The effects of vanadate on mitochondrial respiration and H+ ATPase activity in Saccharomyces cerevisiae were studied. A 50% inhibition of oxygen uptake in isolated mitochondria was produced by 4.4 mM-V2O5. Activity of H+ ATPase in whole mitochondria was inhibited by 50% by 5.5 microM-V2O5, in submitochondrial particles by 55 microM-V2O5; and in the chloroform-released H+ ATPase by 0.5 mM-V2O5. Vanadate was also found to relieve growth inhibition caused by the mitochondrial H+ ATPase inhibitors NN'-decyclohexylcarbodiimide and oligomycin. These results imply that vanadate could affect mitochondrial respiration by interacting with the H+ ATPase in S. cerevisiae.
Keywords:vandate  mitochondria  H+ ATPase  S. cerevisiae
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号