Vanadate inhibition of mitochondrial respiration and H+ ATPase activity in Saccharomyces cerevisiae |
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Authors: | G E Henderson I H Evans I J Bruce |
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Affiliation: | School of Biological Sciences and Environmental Health, Thames Polytechnic, London, U.K. |
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Abstract: | The effects of vanadate on mitochondrial respiration and H+ ATPase activity in Saccharomyces cerevisiae were studied. A 50% inhibition of oxygen uptake in isolated mitochondria was produced by 4.4 mM-V2O5. Activity of H+ ATPase in whole mitochondria was inhibited by 50% by 5.5 microM-V2O5, in submitochondrial particles by 55 microM-V2O5; and in the chloroform-released H+ ATPase by 0.5 mM-V2O5. Vanadate was also found to relieve growth inhibition caused by the mitochondrial H+ ATPase inhibitors NN'-decyclohexylcarbodiimide and oligomycin. These results imply that vanadate could affect mitochondrial respiration by interacting with the H+ ATPase in S. cerevisiae. |
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Keywords: | vandate mitochondria H+ ATPase S. cerevisiae |
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