利用PCR方法检测水稻及其加工产品中转基因水稻

市场上的转基因产品以及深加工产品越来越多,其安全性引起全世界的极大争论,很多国家的检验机构相继开展了转基因产品的检测工作。以转基因水稻为材料,以聚合酶链式反应(PCR)方法为基础,选择适用于转基因食品安全性检验的核酸检测技术,针对转入苏云金杆菌(Bacillus thuringiensis,简写为Bt)基因水稻的Cry1Ac片段进行PCR检测,建立适合转基因水稻的检测方法。该方法简便快速、检测结果与标准及其他文献资料相符。

Detection of transgenic rice gene by polymerase chain reaction methed

Recently, numerous advances have been made in the development of the genetically modified organisms (GMOs) as food. The world general public, however, has shown anxiety over this new technology. Pressure from consumer groups and public demand has demand has led several countries to require labeling for the presence of GMOs in food. Nucleotide-based amplification method is an important system for the identification of genomic modified foods (GMF). Bt Transgenic Rice was used as material to search for feasibility of investigating the safety of GMF by PCR method. The discrimination system for Bt Transgenic Rice from the counterpart of non-GM products and the detection system for correlating marker genes and transgenic are established. The method was easy and fast, and the corresponding results fixed the standard or declared data.