| 有机溶剂和混合油脂对脂肪酶活性恢复的影响 |
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| 引用本文： | 王致禹,陈晓倩,孟庆凤,李春,刘丽波,曲国强,孙雪婷. 有机溶剂和混合油脂对脂肪酶活性恢复的影响[J]. 中国食品学报, 2021, 21(2): 55-62 |
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| 作者姓名： | 王致禹 陈晓倩 孟庆凤 李春 刘丽波 曲国强 孙雪婷 |
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| 作者单位： | 东北农业大学食品学院；乳品科学教育部重点实验室；交铁检验认证中心（成都）有限公司 |
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| 基金项目： | 国家自然科学基金面上项目（31671874）；国家重点研发计划项目（2018YFD0502404）；黑龙江省科学基金项目（C2018022） |
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| 摘 要： | 本文通过多次催化酯交换方法,研究了最优催化条件下脂肪酶活性和结构的关系,以及低活性脂肪酶的活性再生技术.在催化合成1,3-二油酸-2-棕榈酸甘油三酯的研究中,利用荧光光谱和傅里叶变换红外光谱,分析最优条件下不同使用次数脂肪酶的三级与二级结构,研究脂肪酶活性变化与其二级、三级结构之间的关系.使用异丙醇和混合油脂处理脂肪酶...
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| 关 键 词： | 脂肪酶 二级结构 三级结构 活性再生 傅里叶红外光谱 |
Effect of Organic Solvents and Mixed Oils on the Recovery of Lipase Activity |
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| Wang Zhiyu,Chen Xiaoqian,Meng Qingfeng,Li Chun,Liu Libo,Qu Guoqiang,Sun Xueting. Effect of Organic Solvents and Mixed Oils on the Recovery of Lipase Activity[J]. Journal of Chinese Institute of Food Science and Technology, 2021, 21(2): 55-62 |
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| Authors: | Wang Zhiyu Chen Xiaoqian Meng Qingfeng Li Chun Liu Libo Qu Guoqiang Sun Xueting |
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| Affiliation: | (College of Food Science and Technology,Northeast Agricultural University,The Key Laboratory of Dairy Science,Ministry of Education,Harbin 150030;Jiaotie Inpection and Certification Center(Chengdu)Co.,Ltd.,Chengdu 610000) |
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| Abstract: | In this experiment,through multiple catalytic transesterification methods,the relationship between lipase activity and structure under optimal catalytic conditions and the activity regeneration technology of low-activity lipase are studied.Fluorescence spectroscopy and Fourier transform infrared spectroscopy were used to analyze the tertiary and secondary structures of lipases and the relationship between changes in lipase activity and its secondary tertiary structure.Low-activity lipases were restored by treating lipase with isopropanol and mixed fats.The study results showed that with the decrease of lipase activity,the fluorescence intensity of lipase increased gradually,and the amino acid leakage was more and more.In the secondary structure,theα-helix content decreased and theβ-sheet content increased gradually.Through isopropanol treatment,the lipase activity was restored to 75.00%of the original lipase;through the mixed oil treatment,the lipase activity was restored to 96.60%of the original lipase,realizing the purpose of high-efficiency catalytic restoration. |
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| Keywords: | lipase secondary structure tertiary structure activity recovery Fourier transform infrared spectroscopy(FTIR) |
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