杨梅果渣醇提物降糖活性组分筛选及其功能评价

目的:筛选杨梅果渣醇提物降糖活性组分,评价其体内降糖活性.方法:采用AB-8大孔树脂吸附杨梅果渣粗提物,采用不同体积分数的乙醇(10％,30％,50％,70％和90％)洗脱,筛选主要活性成分含量和α-葡萄糖苷酶抑制活性高的组分,采用糖尿病小鼠模型评价其体内降糖活性.结果:30％乙醇洗脱组分(EC30)的总花色苷、总黄酮...

Screening of Hypoglycemic Bioactive Components and Evaluation from Ethanol Extract of Chinese Bayberry Pomace

Objective: To screen the hypoglycemic active constituents from the ethanol extract of Chinese bayberry pomace and evaluate their hypoglycemic activity in vivo. Methods: The AB-8 macroporous resin was used to adsorb the crude extract of Chinese bayberry pomace, and eluted with different concentrations of ethanol(10%, 30%, 50%, 70% and 90%) to screen the constituent with high bioactive component content and α-glucosidase inhibitive activity. The hypoglycemic activity in vivo was evaluated using a diabetic mouse model. Results: The total anthocyanin, total flavonoids and total phenol contents of 30% ethanol elution component(EC30) were significantly higher than other components(P<0.05), which were 5.5, 12.4 folds and 11.4 folds higher than crude extract, respectively. The semi-inhibition concentration IC50,(0.006 ± 0.001)mg/mL] of α-glucosidase in EC30 was significantly lower than that in crude extract IC50,(0.056 ± 0.008) mg/mL](P<0.05). EC30 could alleviate the symptoms of weight loss and reduce fasting blood glucose levels in type Ⅱ diabetic mice;significantly reduce total cholesterol(TC), triglyceride(TG) and low density lipoprotein cholesterol(LDL-c) levels in diabetic mice(P<0.05) and reduce oxidative stress by increasing the activity of antioxidant enzymes such as superoxide dismutase(SOD). etc. In addition, EC30 had a certain repairing effect on liver and kidney cells damaged by diabetic mice. Conclusion: EC30 had the highest bioactive component contents and α-glucosidase inhibitory activity, and it could alleviate type Ⅱ diabetes by improving glucose metabolic pathways and reducing oxidative stress in mice.