表面增强拉曼光谱法快速检测减肥类保健食品中的比沙可啶

目的 建立表面增强拉曼光谱法(surface enhanced Raman spectroscopy,SERS)快速测定减肥类保健食品中比沙可啶的分析方法.方法 激发波长785 nm±0.5 nm,光谱覆盖范围为300～2300 cm-1,光谱分辨率为4～10 cm-1,拉曼光谱仪进行测定,最后采用高效液相色谱法(hi...

Rapid determination of bisacodyl in weight-loss functional foods by surface enhanced Raman spectroscopy

Objective To establish a rapid method for the determination of bisacodyl in weight-loss functional foods by surface enhanced Raman spectroscopy(SERS).Methods The excitation wavelength of the experiment was 785 nm±0.5 nm,the spectral coverage was 300-2300 cm?1,and the spectral resolution was 4~10 cm?1.Raman spectroscopy was used to determine the content,and high performance liquid chromatography(HPLC)was used for verification finally.Results The linear range of this method was from 0.5 to 5 mg/L,and the limit of detection was 0.2 mg/kg.The good recoveries were in range of 77.3%?107.2%with relative standard deviations(RSD,n=6)no more than 6.3%.The results showed no significant difference with those of HPLC.Conclusion The method is rapid and simple.It can be used for the detection of bisacodyl in weight-loss functional foods rapidly.