新疆芜菁多糖含量测定及提取工艺优化

目的探究芜菁水溶性成分多糖的最佳提取工艺及含量测定。方法通过正交实验设计来选择芜菁水溶性成分多糖的最佳水提工艺;以葡萄糖为对照品,使用紫外-分光光度法测定其含量。结果水提的最佳提取工艺:回流时间为2 h,回流温度为90℃,回流次数为3次,料液比为1:30 g/mL。新疆芜菁多糖在0.02~0.10 mg/mL范围内呈良好线性关系,回归方程为Y=0.819X+0.0029, r=0.9995,平均回收率为98.3%,相对标准偏差为2.0%,并测得多糖总含量为8.99%。结论该提取方法方便、合理,满足多糖的分析方法要求;紫外-分光光度法简便易行,精密度、稳定性、重现性均良好。

Determination of polysaccharide content from Brassica rapa L. in Xinjiang and optimization of extraction technology

Objective To explore the optimum extraction technology and content determination method of water-soluble polysaccharide from Brassica rapa L.Methods The optimum water extraction process of water-soluble constituent polysaccharide from Brassica rapa L.was selected by orthogonal experimental design.The content of glucose was determined by ultraviolet spectrophotometry with glucose as reference substance.Results The best extraction technology of water extraction was as follows:reflux time was 2 h,reflux temperature was 90℃,return number was 3 times,and solid-liquid ratio was 1:30 g/mL.The Brassica rapa L.polysaccharide had agood linear relationship in the range of 0.02?0.10 mg/mL.The regression equation was Y=0.819X+0.0029,r=0.9995,the average recovery rate was 98.3%,and relative standard deviation was 2.0%.The total content of polysaccharide was 8.99%.Conclusion The extraction method is convenient and reasonable,meeting the requirement of polysaccharide analysis method.The ultraviolet-spectrophotometry is simple and easy to operate,with good precision,stability and reproducibility.