电感耦合等离子体质谱法测定食品中硒元素的条件优化

目的 优化电感耦合等离子体质谱法(inductively coupled plasma mass spectrometer,ICP-MS)测定食品中硒元素含量的条件,并对河北省食品中硒元素含量情况进行调查分析.方法 选取8种不同基质的国家标准物质作为检测样品对实验条件进行优化,同时选择80Se作为检测目标,采用碰撞模式...

Optimization of determination conditions of selenium in food by inductively coupled plasma mass spectrometry

Objective To optimize the conditions for the determination of selenium in foods by inductively coupled plasma mass spectrometry(ICP-MS),and to investigate and analyze the selenium content in foods in Hebei Province.Methods Eight national reference materials with different matrices were selected as the test samples,and the experimental conditions were optimized.At the same time,80Se was selected as the test target.The selenium content in the samples was determined under the optimized conditions of collision mode,internal standard and 5%isopropanol.By comparing the results,the content of selenium in dietary samples of Hebei province was analyzed to provide guidance for residents to supplement selenium through diet.Results The matrix interference could be effectively reduced by using collision mode and internal standard addition method,and the signal intensity of selenium could be greatly improved by adding isopropanol;the linear range was 5.00-100.00μg/L(r 2=0.999),and the limit of detection was 0.002 mg/kg.The highest content of selenium in nuts and seeds was 1.472 mg/kg,the highest content of soybean was 0.123 mg/kg,and the highest content of duck meat was 0.169 mg/kg.Conclusion Among the 6 kinds of food tested,the selenium content in nuts and seeds,beans and bean products,meat and meat products is relatively high,and the selenium deficient people can increase their consumption appropriately.