不同品温大曲及其曲房空气的细菌群落变化规律和相关性

采用高通量测序技术对中温和高温大曲及其曲房空气细菌群落结构进行研究,同时结合环境因素和大曲细菌群落组成,初步验证环境因素对大曲微生物群落的相关性.高通量测序结果表明:从中温大曲及空气得到33细菌属,其细菌种类数与发酵温度变化呈相反趋势;从高温大曲及空气得到31个细菌属,其细菌种类数在整个发酵过程呈减少趋势;主成分分析发...

Variations and Correlation between Bacterial Communities in Daqu and the Workshop Air at Different Temperatures

The high-throughput sequencing technology was used to study the bacterial community structure of Daqu (the medium-temperature Daqu and the high-temperature Daqu) and the workshop air, and explore the correlation between Daqu and air bacterial communities; at the same time, combined with environmental factors and the composition of Daqu bacterial communities, preliminary verification of environmental factors on Daqu microbial community relevance was performed. The results revealed that 33 bacterial genera were obtained from medium temperature Daqu and air, and the number of bacterial species showed an opposite trend to the change of fermentation temperature; 31 bacterial genera were obtained from high temperature Daqu and air, and the number of bacterial species showed a decreasing trend throughout the fermentation process. Principal component analysis showed that the composition of air bacterial communities in Daqu and workshop air at middle-temperature was basically similar in the early stage of fermentation (except the second stage in fermentation), the composition of the high-temperature Daqu bacterial community was similar throughout the fermentation process, and was similar to that of the workshop air bacterial community in the early fermentation stage. Redundant analysis showed that the 6 factors, including starch content, moisture, acidity, humidity, room temperature, and Daqu temperature, explained 57.41% of the species distribution of Daqu community. The Monte Carlo substitution test found that starch content, moisture and acidity are the main factors affecting the composition of Daqu bacterial community. The dynamic change rule and correlation of bacterial community between Daqu and air during Daqu fermentation were revealed, which provided an important reference for Daqu production and research in the future.