基于纳米酶技术检测柑桔果实中葡萄糖

目的建立纳米酶检测体系快速检测葡萄糖含量的技术。方法采用甲烷氧化细菌的发酵代谢产物甲烷氧化菌素(methanobactin,MB)与Cu²⁺、纳米金(AuNPs)、葡萄糖氧化酶(glucose oxidase,Gox)进行配位结合形成具有过氧化物酶和葡萄糖氧化酶性质的纳米酶检测体系,并对柑桔果实中葡萄糖含量进行测定。结果Gox浓度0.81 U/mL、MB-Cu@AuNPs模拟酶浓度2×10^-5mol/L、对苯二酚浓度5×10^-4mol/L、反应温度60℃、pH 8为最佳检测条件;反应时间5 min,葡萄糖浓度线性范围为1×10^-3-5×10^-2mol/L。结论纳米酶检测体系对柑桔果实中葡萄糖含量的测定具有良好的稳定性,相比较常规快速血糖仪检测速度更快。

Detection of glucose in citrus fruit based on nanometer enzyme technology

Objective To establish a technology for quickly detecting glucose content by a nanometer enzyme detection system.Methods The fermentation metabolite of methane-oxidizing bacteria,methanobactin(MB),was coordinated and combined with Cu2+,AuNPs,and glucose oxidase(Gox)to form a nanometer enzyme detection system with the properties of peroxidase and glucose oxidase,and the content of glucose in mandarine fruit was determined.Results The optimal detection conditions were as follows:Gox concentration 0.81 U/mL,MB-Cu@AuNPs mimic enzyme concentration 2×10?5 mol/L,hydroquinone concentration 5×10?4 mol/L,reaction temperature 60℃,and pH 8.The reaction time was 5 min,and the linear range of glucose concentration was 1×10?3~5×10?2 mol/L.Conclusion The nanometer enzyme detection system had good stability for the determination of glucose content in citrus fruits,and the detection speed is faster than that of the conventional rapid glucose meter.