金属离子对小球藻Chlorella vulgaris生物量和总脂肪酸含量的影响的研究(英文)

本文研究了无光照异养条件下八种金属离子对小球藻Chlorella vulgaris的生物量和总脂肪酸含量(TFAC)的影响.Cu2+,Zn2+是小球藻生长的限制性营养因子,通过添加0.08 mg/L CuSO4·5H2O可使小球藻生物量从1.24 g/L提高到3.60 g/L;添加0.2 mg/LZnSO4·7H2O,可使小球藻生物量从1.71 g/L提高到3.96 g/L.Mg2+缺失条件可得到最高总脂肪酸含量,但是生物量有所减少.Mo6+,Mn2+和Fe3+对小球藻的生物量和油脂含量无明显的影响,说明这些金属离子对小球藻的生长没有特殊的贡献.提高Fe2+的浓度有利于总脂肪酸的提高.添加50 mg/L和100 mg/L的CaCl2·2H2O可分别得到最大生物量和最高总脂肪酸含量.

Effect of Metal Ions on Biomass and Total Fatty Acids Content of Chlorella vulgaris

Eight metal ions were studied for their contributions on biomass and the total fatty acid content (TFAC) of C. vulgaris cultivated heterotrophically in the dark. Cu2+, Zn2+ were limited nutritional factors for the alga growth and the biomass can be improved from 1.24 g/l to 3.60 g/l by adding 0.08 mg/l CuSO4·5H2O. Similarly, the biomass can be improved from 1.71 to 3.96 g/l by adding 0.2 mg/l ZnSO4·7H2O. Under the conditions without adding Mg2+, the highest TFAC can be obtained, but the biomass was decreased. Mo6+, Mn2+ and Fe3+ showed little effect on biomass and the TFAC, indicating that they had no special contribution to the growth of C. vulgaris. Increasing Fe2+ concentration enhanced the TFAC of C. vulgaris. As for Ca2+, the maximal biomass and TFAC can be obtained in the medium containing 50 mg/l and 100 mg/l CaCl2·2H2O respectively.