Oriented immobilization of IgG on hydroxylated Si(001) surfaces via protein-A by a multiple-step process based on a self-assembly approach |
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Authors: | Email author" target="_blank">G?DemirelEmail author M?O??a?layan B?Garipcan M?Duman E?Pi?kin |
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Affiliation: | (1) Department of Chemical Engineering and Division of Bioengineering, Hacettepe University, Beytepe, 06800 Ankara, Turkey |
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Abstract: | The aim of this study was the oriented immobilization of IgG molecules on the silicon surfaces. A multiple-step procedure
was applied for oriented immobilization of IgG in this study. After hydroxylation of the Si(001) surfaces, 3-glycidoxypropyltrimethoxysilane
(GPTS) molecules were self-assembled onto these substrates. Dipping time and GPTS concentration were found to be effected
by on both layer thicknesses and water-contact angles. 2,2′-(ethylenedioxy)diethylamine (EDA) molecules were then covalently
attached to the silicon surface with GPTS molecules. There was no effect of concentration on the formation of EDA molecules
on the surfaces, while EDA deposition increased with the dipping time significantly. Imaging ellipsometry and atomic force
microscopy (AFM) images exhibited aggregate formation at this step. Protein-A molecules were bound to the free amino groups
of EDA molecules on the substrate surface, especially onto the aggregates by using a carbodiimide (i.e., EDAC) as the activating
agent. We were able to immobilize IgG molecules in an oriented form onto the protein-A attached surfaces, especially in the
regions, where EDA aggregates are located. |
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