液体培养条件下产气肠杆菌与阴沟肠杆菌产生物胺交互作用研究

产气肠杆菌和阴沟肠杆菌是发酵香肠中产尸胺和腐胺的主要肠杆菌,常同时被检测分离出来。本研究将产气肠杆菌和阴沟肠杆菌按7:1、5:3、4:4、3:5、1:7的比例混合接种培养,及分别纯菌培养,发现在48h培养过程中,产气肠杆菌有较强的产尸胺能力,而阴沟肠杆菌的产腐胺能力则相对较强;两者混合接种培养时,在产尸胺方面存在明显的协同作用,几乎所用混合体系的尸胺产量都高于两个纯菌体系(P<0.01),在产腐胺方面亦存在协同交互作用,但相对较弱,混合体系的腐胺产量一直介于两个纯菌体系之间。

Effect of Cross-interaction between Enterobacter aerogenes and Enterobacter cloacae on the Production of Biologic Amine

Enterobacter aerogenes and Enterobacter cloacae,as the major cadaverine-and putrescine-producing bacteria,are often isolated simultaneously in the fermented sausages.In this study,E.aerogenes and E.cloacae were inoculated in medium with different proportions during the culture course of 48 h.E.aerogenes revealed strong ability to produce cadaverine,and E.cloacae exhibited a relatively strong ability to produce putrescine.Cadaverine levels exhibited significant changes between the mixed inoculation and the two pure cultures;Two bacteria also had a weak cross-interaction on the production of putrescine.