恩度对横纹肌肉瘤PLA-802细胞生长的抑制作用及其机制

目的探讨人血管内皮抑制素恩度(Endostar)对横纹肌肉瘤PLA-802细胞生长的抑制作用及其机制。方法将常规培养的横纹肌肉瘤细胞PLA-802分为对照组和恩度处理组(不同浓度处理不同时间),采用MTT法检测恩度对细胞生长的影响;流式细胞术检测细胞周期的变化;RT-PCR和Western blot法检测恩度对细胞内血管内皮生长因子(Vascular endothelial growth factor,VEGF)mRNA和蛋白表达的影响;ELISA检测细胞培养液上清中VEGF的生成水平。结果恩度可抑制PLA-802细胞增殖及VEGF的表达和生成,且上述作用具有显著的浓度-时间依赖性。结论恩度能够抑制PLA-802细胞的生长,其机制可能与阻断VEGF的表达相关。

Inhibitory Effect of Endostar on Growth of Rhadomyosarcoma PLA-802 cells and The Relevant Mechanism

Objective To investigate the inhibitory effect of Endostar,an inhibitor of human vascular endothelium,on the growth of rhadomyosarcoma(RMS) PLA-802 cells and the relevant mechanisms.Methods PLA-802 cells cultured by routine method were divided into control and test groups.The cells in control group was untreated,while those in test groups were treated with Endostar at various groups respectively.The effect of Endostar on the growth of PLA-802 cells was determined by MTT method,the change of cell cycle by flow cytometry,the effect of Endostar on expressions of vascular endothelial growth factor(VEGF)mRNA and protein by RT-PCR and Western blot respectively,and the VEGF level in cell culture supernatant by ELISA.Results Endostar inhibited the proliferation of PLA-802 cells as well as expression and production of VEGF,each in a concentration-and time-dependent pattern.Conclusion Endostar can inhibit the growth of PLA-802 cells by a potential mechanism which might be associated with blocking the expression of VEGF.