温度对产甘油假丝酵母产甘油发酵过程的影响

研究了温度对产甘油假丝酵母分批发酵生产甘油及其生长动力学的影响。结果表明:在温度为28—36℃时,由于发酵周期随温度升高而显著缩短,导致细胞生长、甘油合成和葡萄糖消耗的比速率也随温度升高逐渐增加;当温度为32℃时最适于产甘油假丝酵母合成甘油,甘油产量、得率和产率分别达到120.3 g/L,0.523 g/g,1.43 g/(L.h);应用Logistic方程对细胞生长动力学进行了模拟,获得不同温度下的生长动力学参数;根据细胞生长动力学参数,得到28—36℃时甘油分批发酵过程中细胞质量浓度同温度、时间之间的一般关系式,经验证该模型在28—36℃范围内可用于预测不同温度下的细胞生长情况。

Effects of temperature on glycerol production by Candida glycerinogenes

The influence of temperature on growth kinetics of Candida glycerinogenes and glycerol production in the batch fermentation was investigated.The results show that as increasing fermentation temperature from 28 ℃ to 36 ℃,the fermentation time is clearly shortened,while the specific rates of cell growth,glycerol production and glucose consumption are gradually increased.However,it is suitable for glycerol production by Candida glycerinogenes at 32 ℃,and the glycerol mass concentration,yield on glucose and productivity are 120.3 g/L,0.523 g/g and 1.43 g/(L·h),respectively.Logistic equation was successfully applied to estimate the kinetics of cell growth under diverse temperatures.Based on the estimated parameters,the effect of temperature on the kinetics of cell growth was further studied.An equation was developed and applied at 28-36 ℃ to describe the relationship between biomass concentration and fermentation temperature as well as the fermentation time in batch cultures.The experiments validate that this model can predict the growth pattern of the cells very well at 28-36 ℃.