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漆酶处理对杨木APMP木素分子质量的影响
引用本文:崔红艳,刘 玉,杨桂花,王 娟,Lucian A. Lucia. 漆酶处理对杨木APMP木素分子质量的影响[J]. 中国造纸学报, 2011, 26(3): 5-8
作者姓名:崔红艳  刘 玉  杨桂花  王 娟  Lucian A. Lucia
作者单位:1. 山东轻工业学院制浆造纸工程省级重点学科,山东济南,250353
2. 山东轻工业学院制浆造纸工程省级重点学科,山东济南,250353;山东大学微生物技术国家重点实验室,山东济南,250100;美国北卡罗来纳州立大学木材与造纸科学系,美国罗利,NC 27695-8005
3. 美国北卡罗来纳州立大学木材与造纸科学系,美国罗利,NC 27695-8005
基金项目:国家自然科学基金项目(3097232631070525); 山东自然科学基金项目(ZR2010CM065)的资助
摘    要:研究了漆酶和漆酶/介体体系处理对三倍体毛白杨APMP性能的影响.采取酶解-弱酸解两段法分别从APMP原浆和生物处理后的纸浆中分离木素.为了使木素在凝胶渗透色谱流动相四氢呋喃中能完全溶解,对所有试样进行了卞基化处理.利用凝胶渗透色谱分析仪获得了木素的分子质量分布曲线,并计算出数均分子质量和质均分子质量,进而比较了漆酶和漆酶/介体体系处理的效果和差异.

关 键 词:木素  分子质量分布  漆酶  漆酶/介体体系  APMP

The Change of Lignin Molecular Weight of Aspen Alkaline Peroxide Mechanical Pulp Treated by Laccase and Laccase/Mediator
CUI Hong-yan,LIU Yu,YANG Gui-hua,WANG Juan,LucianA.Lucia. The Change of Lignin Molecular Weight of Aspen Alkaline Peroxide Mechanical Pulp Treated by Laccase and Laccase/Mediator[J]. Transactions of China Pulp and Paper, 2011, 26(3): 5-8
Authors:CUI Hong-yan  LIU Yu  YANG Gui-hua  WANG Juan  LucianA.Lucia
Affiliation:CUI Hong-yan1,LIU Yu1,2,3 YANG Gui-hua1 WANG Juan1 Lucian A.Lucia3(1.Shandong Provincial Key Laboratory on Pulp & Paper Science and Technology,Shandong Polytechnic University,Ji'nan,Shandong Province,250353,2.State Key Laboratory for Microbial Technology,Shandong University,250100,3.Department of Forest Biomaterials Science,North Carolina State University,Raleigh,NC 27695-8005)
Abstract:The alkaline peroxide mechanical pulps(APMP) of Triploid of Populus Tomentosa was treated by laccase and laccase-mediator-system(LMS) respectively,the factors affecting physical properties of the pulp were studied.The two-stage method of enzyme-mild acidic hydrolysis was adopted to isolate lignin from the APMP and the modified pulp samples.All the benzoylated lignin samples were dissolved in THF.The molecular weight distributions of lignins were obtained through GPC,the relative number average molecular wei...
Keywords:lignin  molecular weigh distributions  laccase  laccase-mediator-system  APMP  
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