复合法提取高温菜籽粕中蛋白质的研究

以高温菜籽粕为原料,研究了淀粉酶法与碱提法结合提取蛋白质的工艺,其优化条件为:酶解pH7.0,酶解温度50℃,料液比1∶20,加酶量2.5%,酶解时间3 h;碱提pH10.0,碱提温度55℃,碱提时间2 h,料液比1∶12。在此条件下,菜籽蛋白质提取率达到71.63%。经等电点沉淀、70%乙醇脱色及冷冻干燥后,产品呈浅灰色,菜籽蛋白质得率为9.3%,单宁脱除率为56.65%,植酸脱除率为62.77%,硫甙脱除率达93.66%。

Preparation of protein from high temperature rapeseed meal

Protein was extracted from high temperature rapeseed meal by the combined method of enzyme method and alkali extraction. The optimum conditions were determined as follows: enzymolysis pH 7.0, temperature 50 ℃, ratio of material to liquid 1:20, enzyme dosage 2.5%, reaction time 3 h; aqueous alkali pH 10.0, temperature 55 ℃, time 2 h, ratio of material to liquid 1:12. Under the optimal conditions, the extraction rate of rapeseed protein was 71.63%. The extract was precipitated at isoelectric points of rapeseed protein(pH 4.0), and then decolorized by 70% ethanol to obtain the rapeseed protein with light-gray. The yield of rapeseed protein was 9.3%. The removal rate of tannin,phytic acid and glucosinolate was 56.65%, 62.77% and 93.66%,respectively.