| 反相液相色谱法测定7-羟基-3,4-二氢-2(1H)-喹啉酮的含量 |
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| 引用本文： | 颜秋梅,何斌,潘富友. 反相液相色谱法测定7-羟基-3,4-二氢-2(1H)-喹啉酮的含量[J]. 精细化工中间体, 2008, 38(6) |
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| 作者姓名： | 颜秋梅 何斌 潘富友 |
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| 作者单位： | 浙江工业大学化材学院,浙江,杭州,310014;台州学院医药化工学院,浙江,台州,317000 |
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| 基金项目： | 浙江省自然科学基金资助项目 |
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| 摘 要： | 建立了反相液相色谱测定7-羟基-3,4-二氢-2(1H)-喹啉酮含量的方法。采用shim-pack C18柱(5μm,4.6mmi.d.×250mm),流动相为v(甲醇)∶v[缓冲溶液(含事先配制好的0.075mol/L的磷酸二氢铵,磷酸调至pH=3)]=50∶50,流速为0.8mL/min,检测波长为250nm。外标法定量,7-羟基-3,4-二氢-2(1H)-喹啉酮1～100μg/mL浓度范围内呈良好的线性关系,线性回归系数r为0.9997,平均回收率为99.26%,RSD=1.17%(n=9)。
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| 关 键 词： | 反相液相色谱 7-羟基-34-二氢2(1H)-喹啉酮 含量测定 |
Determination of 7-Hydroxy-3,4-dihydro-2(1H)quinolinone by RP-HPLC |
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| YAN Qiu-mei,HE Bin,PAN Fu-you. Determination of 7-Hydroxy-3,4-dihydro-2(1H)quinolinone by RP-HPLC[J]. Fine Chemical Intermediates, 2008, 38(6) |
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| Authors: | YAN Qiu-mei HE Bin PAN Fu-you |
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| Affiliation: | YAN Qiu-mei1,HE Bin1,PAN Fu-you2 (1. College of Chemical Engineering , Materials Science,Zhejiang University of Technology,Hangzhou 310014,China,2. School of Pharmaceutical , Chemical Engineering,Taizhou University,Taizhou 317000,China) |
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| Abstract: | A RP-HPLC method was established for the determination of 7-hydroxy-3,4-dihydro-2(1H)quinolinone. The HPLC system was as follow: shim-pack C18 column (5 μm, 4.6 mm i.d.×250 mm), v (methanol)∶ v[buffer(0.075 mol/L ammonium dihydrogen phosphate, ajusted to pH=3.0 with phosphoric acid)] =50∶50 as mobile phase at flow rate of 0.8 mL/min. The detection wavelength was at 250 nm. The calibration curve was linear from 1.00 to 100.00 μg/mL, the regression coefficient was 0.999 7 and the average recovery of the method was 99.26%, RSD was 1.17% (n=9). |
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| Keywords: | RP-HPLC 7-hydroxy-3 4-dihydro-2(1H)- quinolinone determination |
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