重组巴斯德毕赤酵母工程菌发酵生产藻蓝蛋白的研究

探讨重组巴斯德毕赤酵母工程菌发酵生产藻蓝蛋白的特性,以期能够通过基因工程和发酵工程技术大规模的生产活性重组藻蓝蛋白。先用甘油作碳源培养重组巴斯德毕赤酵母工程菌,达到一定生物量后,再用甲醇诱导外源重组藻蓝蛋白基因的表达,并测定了甘油浓度、细胞干重、细胞光密度和藻蓝蛋白含量的变化。结果表明:所获得的细胞干重最高达41.93g/L,细胞光密度最高为182.77,藻蓝蛋白的最高产量为61.20mg/L,分泌到胞外的藻蓝蛋白的最高产量为24.32mg/L。该研究为实现藻蓝蛋白的产业化生产奠定了良好的基础。

Studies on the Production of Phycocyanin by Recombinant Pichia Pastoris

In order to carry out large scale production of phycocyanin by gene and fermentation engineering, the characters of the phycocyanin production by recombinant pichia pastoris were studied in this paper. After the high biomass of recombinant pichia pastoris was obtained using glycerol as carbon source, and then the phycocyanin gene was induced to express using methanol as carbon source, the glycerol concentration, cell dry weight, cell optical density and the content of phycocyanin were detected. The results indicated that the highest cell dry weight, cell optical density, phycocyanin content and extracellular phycocyanin content obtained were 41.93 g/L,182.77, 61.20 mg/L and 24.32 mg/L, respectively. These lay good foundation for the industrial production of phycocyanin by gene engineering and fermentation technology.