DNA计算中荧光技术的应用及其发展

DNA计箅作为前沿科学研究的重点和热点.已经从简单发展为复杂,从理论转化为应用.在这一过程中,反应速度快、变化灵敏的荧光标记技术发挥了重要的作用.文中围绕DNA计算和荧光标记技术两个方面进行说明.一方面,对近年来DNA计算中荧光技术的应用进行了总结:(1)荧光标记的表面计算;(2)与某些切技术相结合的荧光检测;(3)与DNA链置换相结合的荧光技术;(4)与基因沉默技术相结合的荧光DNA逻辑门;(5)与DNA自组装立体结构相结合的荧光技术;(6)与DNA变构相结合的荧光技术.另一方面,介绍了几种近年来发展起来的新型荧光技术:(1)荧光信号识别放大技术;(2)与磁珠技术相结合的荧光技术;(3)与PH值变化相结合的DNA荧光技术;(4)与miRNAs检测相结合的荧光技术.在今后的研究中,只有将这两者紧密结合,才能发挥DNA计算天然的优势.

Development and Application of Fluorescence Technology in DNA Computing

DNA computing,as a focus of scientific fields,has developed from simple to complex,from theory to application.In this course,the fluorescence,which can be handled easily and has a sensitive respond,plays an important role.This paper pays more attentions to DNA computing and fluorescence.On one hand,major applications of fluorescence in DNA computing are summed up.There are several aspects:(1)surface computing labeled by fluorescence.(2)The fluorescence detecting combined with enzyme cutting.(3)The fluorescence technology used in DNA hybridization and releasing.(4)The fluorescence logical gate based on RNAi.At first,the expression of fluorescence proteins is controlled by RNAi.(5)The fluorescence technology used in DNA self-assembly.(6)The fluorescence technology based on DNA transforming.On the other hand,several novel methods of fluorescence are introduced as following:(1)Signal amplification of fluorescence molecular beacons.(2)The fluorescence technology associated with magnetic beads.(3)The fluorescence technology changing with PH.(4)Detecting miRNAs with fluorescence technology.Only combine these two aspects into whole,most of the advantages of DNA computing can be taken used entirely.