|
|||||
|
|
| 不同来源苦荞中芦丁和槲皮素的含量测定 | |
| 引用本文： | 宾婕,刘洁,陈克嶙,周劲,郭荣富,郭亚东. 不同来源苦荞中芦丁和槲皮素的含量测定[J]. 现代食品科技, 2011, 27(1): 117-119 |
| 作者姓名： | 宾婕 刘洁 陈克嶙 周劲 郭荣富 郭亚东 |
| 作者单位： | 1. 昆明医学院药学院,云南昆明,650500 2. 云南农业大学动物科技学院,云南昆明,650201 3. 云南侨通包装印刷有限公司,云南昆明,657000 |
| 基金项目： | 云南侨通包装印刷有限公司资助项目(kx140564) |
| 摘 要： | 建立了一种苦荞中芦丁和槲皮素含量的测定方法.以超声法进行提取,并分别以芦丁和槲皮素为标品,采用岛津ODS柱(4.6mm×150mm,5μm),以乙腈-水(含2%的冰醋酸)为流动相,梯度洗脱,流速:1 mL·min-1,检测波长:360nm.芦丁进样量在0.023～1.150μg范围内(r=0.9996),槲皮素进样量在... |
| 关 键 词： | 苦荞 芦丁 槲皮素 高效液相色谱 含量测定 |
| 收稿时间： | 2010-09-08 |
Determination of the Contents of Rutin and Quercetin in Buckwheat from Different Sources |
|
| BIN Jie,LIU Jie,CHEN Ke-lin,ZHOU Jing,GUO Rong-fu and GUO Ya-dong. Determination of the Contents of Rutin and Quercetin in Buckwheat from Different Sources[J]. Modern Food Science & Technology, 2011, 27(1): 117-119 | |
| Authors: | BIN Jie LIU Jie CHEN Ke-lin ZHOU Jing GUO Rong-fu GUO Ya-dong |
| Affiliation: | BIN Jie1,LIU Jie1,CHEN Ke-lin2,ZHOU Jing3,GUO Rong-fu2,GUO Ya-dong1(1.School of Pharmacy,Kunming Medical University,Kunming 650500,China)(2.Institute of College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)(3.Yunnan Overseas Package Printing Co.,Ltd,Kunming 657000,China) |
| Abstract: | A new method for determination of rutin and quercetin contents in buckwheat was developed in this paper.The extracts from buckwheat by Ultrasonic extraction was analyzed on a Shimadzu ODS column(4.6 mm×150 mm,5 μm)with a mobile phase of acetonitrile-water(containing 2% glacial acetic acid) for gradient elution at 360nm.And the flow rate was 1.0 mL/min.The linear ranges of rutin and quercetin concentrations were found within 0.023～1.150 μg(r=0.9996) and 0.02～0.60 μg(r=0.9999),respectively.The average recoveryies of rutin and quercetin were 98.5% and 95.8%,respectively.The method was simple,accurate and showed good reproducibility,which was suitable for the determination of rutin and quercetin contents in buckwheat. |
| Keywords: | buckwheat rutin quercetin HPLC assaying |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《现代食品科技》浏览原始摘要信息 | |
| 点击此处可从《现代食品科技》下载免费的PDF全文 | |