Assembly of a protein "brush" by end-grafting titin fragments to liposomes |
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Authors: | Chami Mohamed Franke Barbara Mayans Olga Kreplak Laurent |
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Affiliation: | Center for Cellular Imaging and Nanoanalytics (C-CINA), Biozentrum, University of Basel, Switzerland. |
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Abstract: | Titin is a giant filamentous protein of striated muscle composed of >300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features. |
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