Effects of suni-bug (<Emphasis Type="Italic">Eurygaster</Emphasis> spp.) damage on size distribution of durum wheat (<Emphasis Type="Italic">Triticum durum</Emphasis> L.) proteins |
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Authors: | Burcu Olanca Dilek Sivri Ozay Hamit Koksel |
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Affiliation: | (1) Food Engineering Department, Hacettepe University, 06800 Beytepe/Ankara, Turkey |
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Abstract: | Wholemeal samples were obtained from five durum wheat cultivars at two different bug (Eurygaster spp.) damage levels (medium and high damage). The samples were incubated (60 and 120 min) and used in size exclusion high
performance liquid chromatography (SE-HPLC) analyses. The results showed that the amount of larger polymeric protein (TP1)
and smaller polymeric protein (TP2) obtained from total (sodium dodecyl sulfate soluble) proteins decreased significantly
in the bug-damaged samples, while the amount of total larger monomeric proteins (TP3) increased. The polymeric/monomeric protein
ratio of all cultivars decreased at 60 min of incubation with increasing damage level. For all cultivars, the ratio of unextractable
polymeric protein (UPP%) significantly decreased at 60 min of incubation except cv. Diyarbakir. The results suggested that
bug protease caused depolymerization and/or disaggregation of polymeric proteins to lower their average molecular size. The
changes in protein structure as determined using SE-HPLC supported by the decreases in gluten content and gluten index values
which decreased with suni-bug damage. Deteriorative effects of bug damage on durum wheat quality were found to be quite similar
to those on bread wheats. |
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Keywords: | Durum wheat Suni-bug Eurygaster spp SE-HPLC Unextractable polymeric protein (UPP%) |
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