Purification of two bacteriocins produced by Enterococcus faecalis DBFIQ E24 strain isolated from raw bovine milk |
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| Authors: | Ricardo M. Manzo María de las Mercedes Cardoso Georgina G. Tonarelli Arturo C. Simonetta |
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| Affiliation: | 1. Cátedras de Microbiología y Biotecnología, Departamento de Ingeniería en Alimentos, Facultad de Ingeniería Química (F.I.Q.), Universidad Nacional del Litoral (U.N.L.), Santa Fe, Argentina;2. Departamento de Química Orgánica, Universidad Nacional del Litoral (U.N.L.), Ciudad Universitaria, Santa Fe, Argentina |
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| Abstract: | Enterococcus faecalis DBFIQ E24 was formerly characterised as a producer of antibacterial and antifungal substances. This strain is vancomycin sensitive, nonhaemolytic and gelatinase negative. SDS‐PAGE revealed the presence of an active band located between 1060 and 3500 Da. After the application of a three‐step purification procedure, two antimicrobial peptides were isolated. One of them is mostly active against Bacillus cereus with a molecular mass of 1364 Da, and the other one with a molecular mass of 1686 Da is mainly active against Escherichia coli. These results confirm the technological potential of E. faecalis DBFIQ E24 strain as a GRAS food biopreservative. |
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| Keywords: | Enterococcus faecalis strain Enterocin Purification Food biopreservative Foodborne bacteria |
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