| 尿素包合结合柱层析法制备大豆脱臭馏分中的维生素E |
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| 引用本文： | 陈立国 李岩华 刘程惠 朱靖博. 尿素包合结合柱层析法制备大豆脱臭馏分中的维生素E[J]. 大连轻工业学院学报, 2007, 26(2): 124-127 |
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| 作者姓名： | 陈立国 李岩华 刘程惠 朱靖博 |
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| 作者单位： | [1]大连工业大学生物与食品工程学院,辽宁大连116034 [2]大连民族学院生命科学学院,辽宁大连116600 |
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| 摘 要： | 采用尿素包合法对大豆脱臭馏分中的天然维生素E进行预浓缩，并以KOH滴定值为指标，评价单因素平行试验包合脂肪酸的效果．在最佳尿素包合工艺条件下．维生素E的含量可从原来的3％提高到15％，回收率76．1％．然后，采用硅胶层析柱对预浓缩的维生素E进行分离制备，经HPLC检测，维生素E的纯度达到85％以上，回收率为89．7％．
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| 关 键 词： | 维生素E 硅胶柱层析 尿素包合 大豆脱臭馏分 |
| 文章编号： | 1005-4014（2007）02-0124-04 |
| 修稿时间： | 2006-12-27 |
Preparation of Vitamin E from Soybean Oil Deodorizer Distillate by Urea Inclusion and Column Chromatography |
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| CHEN Li-guo, LI Yan-hua, LIU Cheng-hui, ZHU Jing-bo. Preparation of Vitamin E from Soybean Oil Deodorizer Distillate by Urea Inclusion and Column Chromatography[J]. Journal of Dalian Institute of Light Industry, 2007, 26(2): 124-127 |
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| Authors: | CHEN Li-guo LI Yan-hua LIU Cheng-hui ZHU Jing-bo |
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| Abstract: | Using urea as inclusion material, the nature vitamin E was pre-concentrated from soybean oil deodorizer distillation and the efficiency of embedding fatty acid was evaluated through single-factor experiments with KOH titer value as the index. The Vitamin E content was increased from 3% to 15 %, and the recovery yield was 76.1% under the optimal conditions. In the following separation by silica gel chromatography the vitamin E purity was more than 85% detected by HPLC and recovery was 89.7%. |
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| Keywords: | vitamin E silica gel chromatography urea inclusion deodorizer distillate |
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