酵素中具有抑菌能力乳酸菌的筛选及鉴定

该研究探讨了从酵素中分离纯化具有抗菌性能的12株乳酸菌,对其进行生理生化实验和API鉴定。利用牛津杯法对阪崎大肠杆菌、鼠伤寒沙门氏菌和宋内志贺氏菌、大肠杆菌、金黄色葡萄球菌进行抑菌特性研究,并探讨了乳酸菌是否产生物胺能力和偶氮还原酶。结果表明,酵素源的12株乳酸菌对肠道致病菌均具有不同程度的抑菌活性,其抑菌圈直径为14~22 mm,对大肠杆菌的抑菌效果较好,其抑菌圈直径为15~23 mm,对金黄色葡萄球菌的抑菌圈直径为14~24 mm,12株乳酸菌都不具有产生物胺和偶氮还原酶的能力。通过生理和API生化鉴定确定XZJ003、XZJ006、XZJ015均为副干酪乳杆菌,通过16S rDNA和同源性确证XZJ015为副干酪乳杆菌,该研究结果对今后益生菌的深入研究和功能性食品的开发具有一定的作用。

Screening and Identification of Lactic Acid Bacteria with Antimicrobial Activity in Fruit Ferments

Twelve lactic acid bacteria (LAB) strains with antimicrobial activity were isolated and purified from fruit ferments. These LAB strains were subjected to physicochemical tests and analytical profile index (API) identification, and the Oxford cup assay was used to assess their antimicrobial activities against Escherichia coli sakazakii, Salmonella typhimurium, Shigella sonnei, Escherichia coli, and Staphylococcus aureus. In addition, they were tested for the ability to produce biogenic amines and azoreductases. All 12 LAB exhibited varying degrees of antimicrobial activity against enteropathogenic bacteria, and the diameters of their zone of inhibition (ZOI) ranged from 14 to 22 mm. The LAB were especially effective against E. coli and S. aureus, as their ZOI diameters for these bacteria were 15~23 mm and 14~24 mm, respectively. None of the 12 LAB had the ability to produce biogenic amines or azoreductases. Through physiological and API identification, it was determined that XZJ003, XZJ006, and XZJ015 were all Lactobacillus paracasei. XZJ015 was also identified as L. paracasei through 16S rDNA sequencing and sequence homology analysis. The findings of this study will contribute to future in-depth studies on beneficial bacteria and the development of functional foods.