Continuous biogenic hydrogen production from dilute acid pretreated algal hydrolysate using hybrid immobilized mixed consortia |
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Authors: | Gopalakrishnan Kumar Periyasamy Sivagurunathan Parthiban Anburajan Arivalagan Pugazhendhi Ganesh D Saratale Chang-Su Choi Sang-Hyoun Kim |
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Affiliation: | 1. Department of Environmental Engineering and Science, Daegu University, Jillyang, Gyeongsan, Gyeongbuk 38453, Republic of Korea;2. School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea;3. Center for Materials Cycles and Waste Management Research, National Institute for Environmental Studies, Tsukuba, Japan;4. Department of Civil Engineering, Daegu University, Gyeongsan, Gyeongbuk 38453, Republic of Korea;5. Department of Food Science & Biotechnology, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeongg 10326, Republic of Korea;6. Dai Ho Industry CO., LTD., Chungnam 32925, Republic of Korea |
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Abstract: | This study investigated the bioconversion of dilute acid (2% H2SO4) pretreated red algae (Gelidium amansii) hydrolysate into H2 by anaerobic fermentation in a continuous stirred tank reactor under mesophilic conditions using hybrid immobilized cells as microbial catalyst. Two different hydraulic retention times (HRT) of 24 h and 16 h with a feed concentration of 15 g/L hexose equivalent have been investigated over 85 days of operation to evaluate H2 production performance and stability of the continuous system. The highest hydrogen production rate (HPR) and hydrogen yield (HY) of 2.7 L/L/d and 1.3 mol/mol substrate hexoseadded was achieved at 24 h HRT, while further operation at 16 h HRT led to a significant drop in the hydrogen production with a HPR and HY values of 1.8 L/L/d and 0.7 mol/mol substrate hexoseadded, respectively. The bacterial community analysis characterized by 454 pyrosequencing revealed that the changes in HRT significantly influence the composition of the dominant microflora. At longer HRT (24 h), the phyla Firmicutes was abundant over 98%, whereas at shorter HRT (16 h), Proteobacteria being the dominant populations with 84%. These outcomes suggested that controlling appropriate HRT is prerequisite for efficient hydrogen production. |
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Keywords: | Bioconversion Hydrogen production Red algae Hydrolysate Immobilized cell system |
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