Synthesis and Characterization of Novel Cationic Lipids Derived from Thio Galactose

Two double chain cationic lipids QAS C _n -2-S (n = 12, 14) derived from thio galactose and carbamate-linkage tertiary amine were synthesized and their structures were confirmed by MS, TOF-MS, ¹H NMR and ¹³C NMR. The QAS C₁₂-2-S revealed superior surface activity compared with QAS C₁₄-2-S with lower CMC and γ_CMC. Though Lipo C₁₂-2-S displayed large average particle-size with high polydispersity, positive charged Lipo C _n -2-S can be combined with the negative charged DNA, also negatively stained TEM images confirmed the formation of vesicles. All the above prove that the Lipo C _n -2-S is helpful for gene transfection.