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Application of a microbial sensor for determination of short-chain fatty acids in raw milk samples.
Authors:H Ukeda  G Wagner  G Weis  M Miller  H Klostermeyer  R D Schmid
Affiliation:GBF-Gesellschaft für Biotechnische Forschung, Department of Enzymtechnology, Braunschweig, Federal Republic of Germany.
Abstract:A microbial sensor system, based on the use of immobilized Arthrobacter nicotiana and an oxygen electrode, was applied to determine free short-chain fatty acids in raw milk samples and the result was compared with gas chromatography (GC) and a titrimetric method. The sensor response was linearly related to the concentration of short-chain fatty acids obtained by GC (n = 10, r = 0.92) and to the total concentration of free fatty acids obtained by titrimetric measurement (n = 10, r = 0.78). This result suggests that the present microbial sensor can selectively determine free short-chain fatty acids in raw milk samples and may be useful as a very fast detection method of rancidity in milk.
Keywords:
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