| 废弃蓖麻基润滑油高效降解茵的分离与鉴定 |
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| 引用本文： | 刘亚琼,王昌禄,李风娟,叶峰.废弃蓖麻基润滑油高效降解茵的分离与鉴定[J].天津轻工业学院学报,2012(3):15-20. |
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| 作者姓名： | 刘亚琼 王昌禄 李风娟 叶峰 |
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| 作者单位： | [1]食品营养与安全教育部重点实验室,天津科技大学食品工程与生物技术学院,天津300457 [2]南开大学蓖麻工程研究中心,天津300071 |
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| 基金项目： | 天津市科技兴海项目资助（KJXH2011-06） |
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| 摘 要： | 从内蒙古某蓖麻榨油车间排污口取样，经分离，筛选出茵株M-4，通过对该菌株茵落形态及18SrDNA基因扩增序列分析，鉴定为链格孢属．菌株M-4降解废弃蓖麻基润滑油基础油结果表明，降解7d后，采用气相色谱法（GC）进行定量分析，降解率达71．3％．对菌株生长特性研究表明，在相同接种量下，菌株M-4在含废弃蓖麻基润滑油12％的培养基中仍能较好地生长，并将其降解成污泥状，表明菌株M-4能在含油量较高的环境中良好生长，具有研发应用前景．
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| 关 键 词： | 生物降解 蓖麻基润滑油 降解菌 环境修复 |
Isolation and Identification of Waste Castor-based Oil-highly Efficient Degrading Strains |
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| LIU Yaqiong,WANG Changlu,LI Fengjuan,YE Feng.Isolation and Identification of Waste Castor-based Oil-highly Efficient Degrading Strains[J].Journal of Tianjin University of Light Industry,2012(3):15-20. |
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| Authors: | LIU Yaqiong WANG Changlu LI Fengjuan YE Feng |
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| Affiliation: | 1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science ＆ Technology, Tianjin 300457, China; 2. Nankai University Engineering Research Center for Castor, Tianjin 300071, China) |
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| Abstract: | M-4 strain which was screened out of the sewage and sludge in a castor oil refinery （Inner Mongolia, China） belongs to Alternaria alternata sp., and was identified by 18S rDNA gene sequence analysis. Degradation test of cas- tor based lubricant base oil showed that after 7 days＇ work, the degradation rate of M-4 strain analysed with GC was 71.3%. Studies of the growth characteristics of M-4 have shown that, in the case of the same inoculum, M-4 strains grew well in the concentration of 120 g/L waste castor oil-based mineral medium and the caster oil was degraded into the form of sludge. The results showed that M-4 strain can grow well in high content of oil and has remarkable restoration effect on the environment polluted by the castor based lubricants, which indicaties that it will have good prospect in application. |
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| Keywords: | biodegradable castor based lubricants degrading bacteria environmental remediation |
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