Antioxidative Capacity of Irbesartan

The author investigated the antioxidant effect of irbesartan[2-butyl-3-({4-[2-(2H-1,2,3,4-tetrazol-5-yl)phenyl]phenyl}methyl)-1,3-diazaspiro[4.4]non-1-en-4-one],an angiotensin receptor biocker(ARB),on the oxidation of erythrocytes induced by 2,2-[azobis(2-amidinopropane)hydrochloride](AAPH)and H2O2.The value of half concentration(IC50)of irbesartan to scavenge radicals was measured by reacting it with 2,2-[azinobis(3-ethylbenzothiazoline-6-sulfonate)radical cation](ABTS+).Activities of the catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and the content of malondialdehyde(MDA)in liver tissue and blood serum of normal-rats were measured by means of spectrophotometry to study the antioxidation function of irbesartan.Results of experiments show that irbesartan can scavenge ABTS+ and superoxide radicals effectively as well as inhibit AAPH-,H2O2-induced hemolysis of erythrocytes,Irbesartan can also increase the activities of GSH-Px,SOD,CAT and decrease the content of MDA of normal rats.So irbesartan is a good antioxidant.