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Intestinal Transport and Biotransformation of Resibufogenin and Cinobufagin in Chan Su via HPLC/APCI-MSn |
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| Authors: | HAN Tian-jiao WANG Qing SONG Feng-rui LIU Zhong-ying LIU Zhi-qiang LIU Shu-ying |
| Affiliation: | 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, P R. China;Graduate School of the Chinese Academy of Sciences, Beijing 100049, P R. China 2. Department of Endocrinology of China-Japan Union Hospital of Jilin University, Changchun 130033, P. R China 3. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, P R. China 4. School of Pharmacy, Jilin University, Changchun 130033, P R. China |
| Abstract: | In vitro models of human colon carcinoma cell line(Caco-2 cell monolayer) and human intestinal bacteria were used to investigate the intestinal transport and biotransformation of resibufogenin and cinobufagin in Chan Su by HPLC/APCI-MS<'n>. The experimental results of Caco-2 cell monolayer demonstrate that the apparent permeability cocfficients(P<,app>) of resibufogenin and cinobufagin are higher than 10<'-6> cm/s, which indicates that both resibufogenin and cinobufagin have a good absorption in the small intestine. And the biotransformation result of human intestinal bacteria shows that resibufogenin has been transformed to 3-epiresibufogenin and cinobufagin has been transformed to 3-epicinobufagin, deacetyicinobufagin and 3-epideacetycinobufagin, respectively. |
| Keywords: | Chan Su Resibufogenin Cinobufagin Caco-2 cell monolayer Human intestinal bacteria |
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