Characterization of an oil-degrading Microcoleus consortium by means of confocal scanning microscopy, scanning electron microscopy and transmission electron microscopy |
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Authors: | Diestra Elia Solé Antonio Martí Mercedes Garcia de Oteyza Tirso Grimalt Joan O Esteve Isabel |
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Affiliation: | Department of Genetics and Microbiology, Autonomous University of Barcelona, 08193 Bellaterra, Spain. elia.diestra@uab.es |
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Abstract: | A consortium of microorganisms with the capacity to degrade crude oil has been characterized by means of confocal laser scanning microscopy (CLSM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The analysis using CLSM shows that Microcoleus chthonoplastes is the dominant organism in the consortium. This cyanobacterium forms long filaments that group together in bundles inside a mucopolysaccharide sheath. Scanning electron microscopy and transmission electron microscopy have allowed us to demonstrate that this cyanobacterium forms a consortium primarily with three morphotypes of the heterotrophic microorganisms found in the Microcoleus chthonoplastes sheath. The optimal growth of Microcoleus consortium was obtained in presence of light and crude oil, and under anaerobic conditions. When grown in agar plate, only one type of colony (green and filamentous) was observed. |
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Keywords: | Microcoleus consortium confocal laser scanning microscopy transmission electron microscopy scanning electron microscopy crude oil |
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