响应曲面法优化短双歧杆菌Bifidobacterium breve A04培养基

本文通过响应曲面法(RSM)达到优化双歧杆菌培养基组分的目的。以TPYG培养基为基础培养基,采用FFD(factionalfactorialdesign)实验设计法,筛选出三种最佳生长强化因子葡萄糖、酵母粉和初始pH值;通过快速登高法摸索出生长强化因子的最佳浓度范围为:葡萄糖5.0g/L,酵母膏3.01g/L以及初始pH6.0;利用旋转中心组分设计法(RCD)和响应曲面法(RSM)确定关键组分的最佳浓度并进行了验证。最终优化培养基组分为葡萄糖5.194g/L,酵母膏2.829g/L,低聚果糖2g/L,西红柿汁50ml,肝浸液5g/L,Tween801ml,半胱氨酸盐酸盐0.3g/L,pH6.463。最终优化出的培养基获得的菌体干重达到4.462g/L,比优化前3.16g/L提高了1.43倍。

Medium Optimization of Cell Production of B. breve A04 Using Response Surface Methodology

Response surface methodology (RSM) was used to optimize the medium components of B.breve A04. In the firststep of the optimization, a factional factorial design was used to evaluate the effects of nine factors. The results showed that threefactors, including glucose, yeast extract and initial pH had significant effects on bacteria dry weight. Secondly, the path ofsteepest ascent was used to gain the optimal region of the three factors. At last, the concentrations were optimized using CCRD(Central Composite Rotable Design) and RSM analysis. As a result, the contents were obtained as follows: (/L): glucose5.194g,yeast extract2.829g, FOS 2g, tomato juice50ml, liver infusion 5g, Tween80 1ml, cystein-HCl 0.3g, and pH6.463. Final dryweight of biomass was obtained as 4.462g/L, 1.43 higher than before 3.16 g/L.