Effects of lead toxicity on the total chlorophyll content and growth changes of the aquatic plant Ceratophyllum demersum L.

This paper reports results of a laboratory-scale experiment conducted to determine the effects of different initial concentrations of a toxic heavy trace metal (lead) on the total chlorophyll (a + b) content of aquatic plant tissues Ceratophyllum demersum L., and also to monitor the visible growth changes during the experiment under different lead concentrations (2, 4 and 6 mg/L), as well as in control plants (without any addition of lead nitrate solution). Lead concentrations in water and plant samples were determined using AAS, where the total chlorophyll content in plant leaf tissues was measured every 15 days during the experimental period using a UV/VIS Spectrophotometer, Model 160A, Shimadzu, with wavelengths of 665 and 649 nm. The results showed that the content of total chlorophyll decreased noticeably, with increasing initial concentrations of lead throughout the exposure time. Decreases in total chlorophyll content in the tissues of C. demersum over the period of the experiment were accompanied by different morphological changes in the plants, depending on the initial lead concentration and exposure duration. These changes in morphology and growth affected the number and length of branches, the number of leaves and the number of leaflets.