| 可逆饱和光转移过程的荧光超分辨显微术 |
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| 引用本文： | 郝翔,匡翠方,李旸晖,刘旭.可逆饱和光转移过程的荧光超分辨显微术[J].激光与光电子学进展,2012(3):38-46. |
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| 作者姓名： | 郝翔 匡翠方 李旸晖 刘旭 |
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| 作者单位： | 浙江大学现代光学仪器国家重点实验室光电显示技术研究所 |
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| 基金项目： | 中央高校基本科研业务费专项资金(2010QNA5 035);浙江省自然科学基金(Y1100408);浙江省教育厅科研项目基金(Y201009976)资助课题 |
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| 摘 要： | 基于可逆饱和光转移过程的荧光超分辨显微技术,从原理上打破了原有的光学远场衍射极限对光学系统极限分辨率的限制,在生物、化学、医学等多个学科拥有广泛的应用前景。回顾了近年来超分辨显微研究的历史,综述了目前常见的几种基于可逆饱和光转移过程的荧光超分辨显微方法,详细描述了各自的技术特点并对比了其优缺点,阐述了相关领域内最新的研究工作进展。
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| 关 键 词： | 显微 超分辨 可逆饱和光转移过程 受激发射损耗显微镜 基态损耗显微镜 饱和图案激发显微镜 饱和结构光照明显微镜 |
Reversible Saturable Optical Transitions Based Fluorescence Nanoscopy |
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| Hao Xiang Kuang Cuifang Li Yanghui Liu Xu.Reversible Saturable Optical Transitions Based Fluorescence Nanoscopy[J].Laser & Optoelectronics Progress,2012(3):38-46. |
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| Authors: | Hao Xiang Kuang Cuifang Li Yanghui Liu Xu |
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| Affiliation: | Hao Xiang Kuang Cuifang Li Yanghui Liu Xu(Institute of Photoelectric Display,State Key Laboratory of Modern Optical Instrumentations,Zhejiang University,Hangzhou,Zhejiang 310027,China) |
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| Abstract: | The reversible saturable optical transitions(RESOLFT) based fluorescence nanoscopy,which fundamentally breaks the barrier of the diffraction limit in the far field,is expected to be widely used in biology,chemistry and medicine domain.This paper reviews the history of fluorescence nanoscopy technology,summarizes some general RESOLFT approaches,and explicitly describes their corresponding characters,especially the advantages and drawbacks.The new improvement in related domains are also shown. |
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| Keywords: | microscopy superresolution reversible saturable optical transitions stimulated emission depletion groud state depletion saturated pattern excitation microscopy saturated structured illumination microscopy |
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