黑木耳化学成分的研究

目的:对黑木耳中的化学成分进行研究。方法:采用硅胶、凝胶等多种色谱方法对黑木耳中的化学成分进行分离和纯化,通过核磁共振色谱和质谱等方法对化合物结构进行鉴定,采用比浊法测定分离纯化后的黑木耳提取物抗血小板凝集活性。结果:从黑木耳的甲醇层提取物中得到3种化合物,通过结构鉴定,分别为尿苷(uridine)、α-D-吡喃葡萄糖(α-D-glucopyranose)、脑苷酯B(cerebroside B)。结论:尿苷、脑苷酯B为首次从黑木耳中分离得到。脑苷酯B对大鼠血小板凝集无抑制作用,尿苷(100μg/mL)对血小板凝集有抑制活性,抑制率为17.9%。

Study on Chemical Composition of Auricularia auricular

【Objective】To study the chemical consitituents of Auricularia auricular.【Method】The compounds were isolated and purified by many kinds of chromatography methods and were identified on the basis of physico-characters and spectroscopic analysis.The method of turbidimetry was used to determine the antiplatelet-aggregating activity of compounds.【Result】Totally,3 kinds of compounds were obtained from the methanol layer extracts of Auricularia auricular and their structures were identified as Uridine,α-D-glucopyranose,and cerebroside B.【Conclusion】Uridine and cerebroside B are isolated from this fungs for the first time.It is suggested that uridine（100μg/mL） inhibited SD-mice platelet aggregation induced by ADP,but the cerebroside B showed no inhibition effect.