| 食用大豆油中转基因成分的检测 |
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| 引用本文： | 邓鸿铃,覃芳芳,郭新东,吴玉銮. 食用大豆油中转基因成分的检测[J]. 中国油脂, 2007, 32(8): 79-81 |
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| 作者姓名： | 邓鸿铃 覃芳芳 郭新东 吴玉銮 |
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| 作者单位： | 广州市产品质量监督检验所食品中心,510110,广州市八旗二马路38号 |
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| 摘 要： | 食用油脂中转基因成分的检测是当前食品检验工作的一个主要方面。针对食用油脂中DNA含量极低、DNA序列片段短、破坏严重的特点,建立了食用油脂中DNA提取方法,通过实时荧光PCR可检测出大豆内源基因(Lectin)以及外源抗除草剂基因EPSPS,为食用油脂进行核酸类生物性检测提供了一种简捷有效的方法。
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| 关 键 词： | 食用油脂 DNA提取 转基因检测 |
| 文章编号： | 1003-7969（2007）08-0079-03 |
| 修稿时间： | 2007-01-10 |
Detection of genetically modified organisms in edible soybean oils |
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| DENG Hong-ling,QIN Fang-fang,GUO Xin-dong,WU Yu-luan. Detection of genetically modified organisms in edible soybean oils[J]. China Oils and Fats, 2007, 32(8): 79-81 |
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| Authors: | DENG Hong-ling QIN Fang-fang GUO Xin-dong WU Yu-luan |
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| Affiliation: | Guangzhou Products Quality Supervision and Testing Institute,510110 Guangzhou,China |
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| Abstract: | In recent years,detecting transgenic genes in edible oils is a major aspect in food detection.According to the feature of low DNA content,short DNA fragment and seriously destroyed DNA,a useful method for extraction DNA from the edible oil samples was established and the extracted DNA could be used as template for PCR.The endogenous genes(Lectin) and transgenic genes(EPSPS) could be detected in the edible oil samples by the real-time PCR method,which provided a simple and effective method for detecting nucleic acid substance in edible oils. |
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| Keywords: | edible oil DNA extraction detection of GMOs |
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