Distribution and elimination of levamisole in eggs and tissues after oral administration to laying hens,determined by LC-MS/MS |
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Authors: | B. Solomun Kolanović N. Bilandžić B. Kos J. Šušković L. Cvetnić I. Varenina |
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Affiliation: | 1. Department for Veterinary Public Health, Laboratory for Residue Control, Croatian Veterinary Institute, Zagreb, Croatia;2. Laboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia;3. Department for Bacteriology and Parasitology, Laboratory for Mastitis and Raw Milk Quality, Croatian Veterinary Institute, Zagreb, Croatia |
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Abstract: | Levamisole was administered to laying hens, and concentrations in eggs and tissues (thigh muscle, breast muscle, liver and kidney) were determined by a newly developed liquid chromatography tandem mass spectrometry method, which allowed trace level quantification of levamisole. The adopted analytical method showed good sensitivity, repeatability and percentage of recovery from spiked matrices. Maximum concentrations of levamisole were found on the first day after the administration (531.1 μg/kg in liver, 164.3 μg/kg in egg yolk, 130.7 μg/kg in kidney, 78.0 μg/kg in breast muscle, 70.7 μg/kg in thigh muscle and 64.0 μg/kg in egg white), after which there is a decline. The compound was rapidly eliminated from eggs, with a half-life of 1.3 days. Elimination appeared to be slower in thigh muscle (3.5 days), breast muscle (3.4 days) and liver (3.3 days). According to this experiment, the levamisole withdrawal periods calculated for eggs, liver, kidney, breast muscle and thigh muscle in laying hens were 14.1, 6.1, >4.0, 14.5 and 13.0 days, respectively. The longest time for levamisole residues to be completely released from tissues was seen in liver samples (37.4 days), followed by thigh muscle, breast muscle and kidney. Elimination from eggs was fastest (16.4 days for levamisole residues to drop below the method quantification limit). |
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Keywords: | Levamisole laying hens eggs tissues withdrawal time LC-MS/MS |
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