木瓜蛋白酶在介孔泡沫材料上的固定及性能研究

以氧化硅介孔泡沫材料(Siliceous mesocellular foam,MCF)为载体对木瓜蛋白酶进行了固定,并对固定化后材料的表征、固定化酶的性能以及酶的稳定性进行了研究.结果表明,木瓜蛋白酶在MCF上能获得较大的固定量(334mg/g MCF)和较高的酶活,固定化酶活力为游离酶的95.3%.固定化酶和游离酶的米氏常数Km值分别为6.99×10-3mol/L和6.71×10-3mol/L,并且相对于游离酶固定化酶的热稳定性和储藏稳定性都有明显改善.MCF是一种良好的木瓜蛋白酶固定载体.

Immobilization of Papain on Siliceous Mesocellular Foam

Siliceous mesocellulax foam(MCF) is employed as carriers in the immobilization of papain, and the properties and the stabilities of the immobilized enzyme axe investigated in detail. The results show that the amount of papain immobilized on MCF reaches 334mg/g MCF and the activity of immobilized papain is 95. 3% of that of the free papain. The Km of immobilized and free papain are 6. 99×10-3 mol/L and 6. 71×10-3mol/L, respectively. The thermal and storage stabilities of immobilized enzyme are improved due to the mieroenvironment arid the shield of the mesopores. MCF can be considered as excellent supports for papain immobilization.