利用甘蔗糖蜜与乳清粉厌氧发酵制备丁二酸

考察了甘蔗糖蜜替代昂贵葡萄糖作为碳源、乳清粉替代大部分酵母粉作为氮源时，对Actinobacillus succinogenes NJ113发酵制备丁二酸的影响。血清瓶厌氧发酵结果证明：对照组（葡萄糖40 g/L）的丁二酸产量仅为26.04 g/L，而以糖蜜为碳源（以总还原糖计算为40 g/L）时，丁二酸产量达到28.27 g/L，比对照组提高了8.57%。在此基础上，以糖蜜为碳源、不同比例的乳清粉和酵母粉为混合氮源发酵制备丁二酸，确定了糖蜜、乳清粉和酵母粉混合使用的最佳浓度分别为40 g/L、8 g/L和2 g/L。此外，在3 L发酵罐体系中添加40 g/L糖蜜、8 g/L乳清粉、2 g/L酵母粉进行发酵试验，实验结果证明：丁二酸终产量达到32.54 g/L，收率达到81.13%。

Preparation of succinic acid using sugarcane molasses and whey powder

he effect of using sugarcane molasses and whey powder to prepare succinic acid by the fermentation of Actinobacillus succinogenes NJ113 was investigated. When the concentration of glucose was 40 g/L, the production of succinic acid reached to 26.04 g/L, and which was 28.27 g/L when using the same concentration of molasses as carbon source. It indicates that the production of succinic acid using sugarcane molasses was improved by 8.57%. When whey powder and extract yeast was used as nitrogen source, the optimal concentrations of whey powder and extract yeast were 8 g/L and 2 g/L respectively. With 40 g/L molasses, 8 g/L whey powder and 2 g/L yeast powder in a 3 L fermenter, the production of succinic acid was 32.54 g/L and the yield of succinic acid reached to 81.13%.