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聚酰胺树脂提取血竭龙血素
引用本文:乐薇.聚酰胺树脂提取血竭龙血素[J].化学与生物工程,2012,29(11):57-60.
作者姓名:乐薇
作者单位:武汉长江工商学院工学院,湖北武汉,430065
摘    要:研究了聚酰胺树脂对血竭中龙血素A和龙血素B的提取效果。采用HPLC进行定量分析,考察了上样方法、洗脱剂体积分数、洗脱流速、树脂使用次数等因素对提取效果的影响,并确定了适宜的梯度洗脱方式。结果表明,将血竭样品用乙酸乙酯溶解后,与聚酰胺树脂混合,然后将溶剂蒸发完全进行吸附,树脂的吸附量为1g·(g树脂)-1。在0.8mL·min-1洗脱流速下,7BV的50%乙醇可梯度洗脱被聚酰胺树脂吸附的龙血素A和龙血素B,解吸率均达91%以上,聚酰胺树脂使用3次后即需再生。该工艺收率高、操作简便,适于血竭龙血素的提取。

关 键 词:聚酰胺树脂  提取  血竭  龙血素

Abstraction of Loureirin A and B in Dragon's Blood by Polyamide Resin
YUE Wei.Abstraction of Loureirin A and B in Dragon's Blood by Polyamide Resin[J].Chemistry & Bioengineering,2012,29(11):57-60.
Authors:YUE Wei
Affiliation:YUE Wei(Engineering Institute of Wuhan Yangtze Business University,Wuhan 430065,China)
Abstract:The abstraction of loureirin A and B in dragon's blood by polyamide resin was studied. The effect of sampling method, eluent volume fraction, eluting velocity, frequency of use of polyamide resin on the abstrac- tion efficiency were investigated,and the quantitative analysis was conducted on HPLC. And the gradient elu- tion way was optimized. Results showed that,the adsorbing capacity of polyamide was 1 g · g-1 resin when the process was as follows.after dissolved with acetic ether, the sample was mixed with polyamide resin,and then the organic solvent was evaporated completely. The loureirin A and B adsorbed by polyamide resin could be gra- diently eluted more than 91% by 7 BV of 50% ethanol at the eluting velocity of 0.8 mL·min-1. The process was fit for the abstraction of loureirin A and loureirin B in dragon's blood with high yield and simple operation.
Keywords:polyamide resin  abstraction  dragon's blood  loureirin
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