A simple and rapid antibody-capture ELISA for the detection of staphylococcal enterotoxin A in food including a simple extraction step |
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| Authors: | TEREZA CRISTINA RM DE OLIVEIRA HEATHER A LEE†† GARY WYATT† ELISA Y HIROOKA MICHAEL RA MORGAN† |
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| Affiliation: | TAM-CCA- Universidade Estadual de Londrina, CP 6001, Londrina, Parunu, Brazil 86051;Food Molecular Biochemistry, Norwich Laboratory, Norwich Reseurch Park, Colney, Norwich NR4 7UA, UK;Department, Institute of Food Research, Norwich Laboratory, Norwich Reseurch Park, Colney, Norwich NR4 7UA, UK |
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| Abstract: | A sensitive and precise ELISA for the detection of staphylococcal enterotoxin A in food has been developed, using specific IgG anti-toxin antibodies purified from immunized rabbits. an antibody capture format was found to be the most useful following cross-reaction and sensitivity studies. the ELISA was used to investigate concentration of staphylococcal enterotoxin A from food samples by polyethylene glycol dialysis. the procedure was shown to be inefficient and highly variable, as well as time-consuming. Direct analysis of aqueous food extracts without a concentration step was found to be possible. With an assay time of 140 min, the ELISA detection limit was 12.5 pg of toxin (corresponding to a sensitivity of 0.5 ngg-1 of food sample. |
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| Keywords: | Contamination food analysis food safety immunoassay trace analysis |
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