HSA微胶粒(SAA)药盒的制备及其99Tcm标记

用生理盐水将20%医用人血清白蛋白(HSA)静脉注射液稀释后,在碱性条件下加热制备HSA微胶粒(SAA),冻干制成药盒;电镜检查显示得到了粒径＜80 nm的SAA,颗粒范围适合于骨髓、淋巴和炎症显像.用Na 99TcmO4溶液对药盒进行标记,标记率＞99%,并可稳定至标记后4 h.小鼠生物分布结果提示,99Tcm-SAA主要从肾脏排泄,60 min时肝、脾和肾放射性较高;家兔显像结果显示给药后30～60 min骨髓可以清晰显影.该SAA药盒标记方法简单,质量稳定,骨髓摄取率高,有望成为一种新的胶体显像剂.

The Preparation of Small Albumin Aggregates(SAA)Kit and Labeling With 99Tcm

A kit for preparing ~(99)Tc~m-SAA(small albumin aggregates) is obtained by heating the HSA solution and freeze-drying the product. The particle size detected by electron microscope is smaller than 80 nm which is suitable for bone marrow, inflammation and lymph imaging. The labeling yield with Na~(99)Tc~mO_4 is more than 99% and the labeling is stable within 4 hours. The biodistribution in mice shows that the ~(99)Tc~m-SAA excretes mostly from the urinary tract. High concentration of radioactivity in liver, spleen and marrow are observed and the bone marrow can be seen clearly from 30 to 60 minute after injection in rabbit imaging. The labeling method is simple,~(99)Tc~m-SAA has desirable stability, higher uptake in marrow and may serve promising as a new colloid imaging agent.