Comparison of HPLC and GLC methodologies for determination of glucosinolates using reference material |
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Authors: | Karel Hrniík J Velíek and Jií Davídek |
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Affiliation: | (1) Institute of Chemical Technology, Department of Food Chemistry and Analysis, Technicka 1905, CR-166 28 Prague 6, Czech Republic, XX |
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Abstract: | The reference material rapeseed of known glucosinolate composition, was analysed by HPLC and GLC and two commercially available
glucosinolates (glucotropaeolin and sinigrin) were used as internal standards. The HPLC method enabled determination of 11
different glucosinolates (as desulphoglucosinolates) that occur in the rapeseed. Only seven glucosinolates (as trimethylsilylated
desulphoglucosinolates) were separated by GLC. The latter method did not allow the determination of methylsulphinylalkyl glucosinolates
(glucoiberin, glucoraphanin and glucoallysin) and did not separate optical isomers of 2-hydroxy-3-butenyl glucosinolate (progoitrin
and epi-progoitrin). Statistical evaluation of data (t-test, F-test) revealed no significant differences between the tested methods at the 95% confidence level. The advantages and disadvantages
of both widely used chromatographic methods are discussed.
Received: 26 May 1997 / Revised version: 11 July 1997 |
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Keywords: | Glucosinolate Rapeseed HPLC GLC |
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