| 两种苦荞黄酮提取方法的优化及含量测定 |
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| 引用本文： | 李欣,王步军. 两种苦荞黄酮提取方法的优化及含量测定[J]. 食品科学, 2010, 31(6): 80-85. DOI: 10.7506/spkx1002-6630-201006018 |
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| 作者姓名： | 李欣 王步军 |
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| 作者单位： | 中国农业科学院作物科学研究所，农业部谷物品质监督检验测试中心 |
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| 基金项目： | 科技部科研院所社会公益研究专项(2004DIB4J145) |
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| 摘 要： | 采用超声波和恒温水浴振荡两种前处理方法，通过单因素和正交试验确定最佳提取方法，并对确定的最佳提取方法进行总黄酮和芦丁含量的回收率、重复性、精密度等验证，初步建立苦荞麦黄酮类化合物的提取测定方法。结果表明：超声波法的最佳提取条件为80% 乙醇溶液、液固比40:1、超声提取30min；恒温水浴的最佳提取条件为80% 乙醇溶液、液固比30:1、60℃提取3h。两种提取方法的最终提取效果差异不显著，而超声波法则大大节省了时间。
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| 关 键 词： | 苦荞麦 总黄酮 芦丁 |
| 收稿时间： | 2009-04-07 |
Optimization of Two Extraction Techniques of Total Flavonoids from Tartary Buckwheat and HPLC Determination Based on the Two Extraction Techniques of Total Flavonoids in Tartary Buckwheat |
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| LI Xin,WANG Bu-jun. Optimization of Two Extraction Techniques of Total Flavonoids from Tartary Buckwheat and HPLC Determination Based on the Two Extraction Techniques of Total Flavonoids in Tartary Buckwheat[J]. Food Science, 2010, 31(6): 80-85. DOI: 10.7506/spkx1002-6630-201006018 |
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| Authors: | LI Xin WANG Bu-jun |
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| Affiliation: | Cereal Quality Supervision and Testing Center, Ministry of Agriculture, Institute of Crop Sciences,Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | Ultrasonic or constant temperature shaking water bath was used for extraction of total flavonoids from tartary buckwheat in the present study.The optimal ultrasonic-assisted and constant temperature shaking water bath extraction procedures were investigated by single factor and orthogonal array methods.Meanwhile,HPLC determination based on the two optimized extraction techniques of total flavonoids in tartary buckwheat was carried out.Results showed that the optimal values of crucial parameters affecting ex... |
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| Keywords: | tartary buckwheat total flavonoids rutin |
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